Optimization of enzyme assisted ultrasonic extraction process for Artemisia argyi and study on its antioxidant and antibacterial activities

Based on the analytic hierarchy process entropy weight method （AHP-EWM） comprehensive scoring system， combined with response surface test design， the enzyme-assisted ultrasound extraction process of Artemisia argyi was optimized. The antioxidant capacity of Artemisia argyi extract was determined using the determination method of DPPH and OH radical scavenging ability. The inhibition zone method and the double dilution method were used to determine the antibacterial ability of Artemisia argyi extract. The results showed that the optimal process parameters for the enzyme assisted ultrasound extraction of Artemisia argyi were cellulase dosage of 3.5%， enzymatic hydrolysis temperature of 55 ℃， enzymatic hydrolysis time of 75 min， and ultrasonic time of 40 min. Under these conditions， the comprehensive score was 96.65±1.44. The IC₅₀ values of Artemisia argyi extract for DPPH and OH radicals were （45.00±1.43） mg/mL、（47.51±1.14） mg/mL， respectively， indicating good antioxidant activity. The diameters of the inhibition zones of Artemisia argyi extract against Staphylococcus aureus， Escherichia coli， and Bacillus subtilis were （15.20±0.40） mm、（14.10±0.20） mm、（13.16±0.18） mm， respectively. The minimum inhibitory concentrations were 0.25， 0.50 and 0.50 g/mL， respectively， indicating good antibacterial activity.