The effect of FgFabD gene on the growth， development and infection of fusarium graminearum
-
-
Abstract
Malonyl-CoA-ACP transacetylase（FabD） catalyzes the conversion of malonyl-CoA to malonyl-ACP， the second step in the fatty acid synthesis pathway， playing a crucial role in the growth of Fusarium graminearum. To reveal the biological function of FgFabD， the wild type strain of Fusarium graminearum PH-1 was used. FgFabD deletion mutants （ΔFgFabD） and complementary strains （ΔFgFabD-C） were constructed by Split-Marker gene knockout technology. PH-1， ΔFgFabD and ΔFgFabD-C were individually inoculated in different cultural conditions to analyze the growth characters， and to reveal the effect of FgFabD on the growth， development， pathogenesis and mycotoxin productions. The results demonstrated that the growth rate and colony phenotype of ΔFgFabD showed no significant difference to wild strain PH-1 in regular medium. In the stress selection medium containing 0.7 mol/L NaCl， 0.03% H2O2， 0.01% SDS and 300 μg/mL Congo-Red， the growth of ΔFgFabD mutant was significantly lower than wild-type strain PH-1. The evidence demonstrated that FgFabD gene played important roles in the formation of cell membrane and cell wall. The conidia production of fusarium graminearum was significantly reduced compared to wild strain PH-1. Inoculation experiments showed that the pathogenicity of the mutant on wheat coleoptiles and wheat heads was significantly lower than that of the wild-type strain. Rice medium inoculation tests further demonstrated that the DON produced by ΔFgFabD was significantly lower than that produced by wild strain. These results demonstrated that FgFabD played key roles in the asexual reproduction， pathogenicity and DON biosynthesis of F. graminearum.
-
-
DownLoad: