Identification and functional study of key lncRNAs regulating the differentiation of porcine skeletal muscle satellite cells

The development of porcine skeletal muscle can directly affect the economic benefits of the livestock industry. Aiming at the problem of porcine skeletal muscle development， the regulatory role of key lncRNAs was explored in the myogenic differentiation of porcine skeletal muscle satellite cells. The RNA-Seq method was used to construct the transcriptional atlas of porcine skeletal muscle satellite cells. The results showed that： 3849 genes were differentially expressed in skeletal muscle cells at different differentiation stages， among which 3557 were mRNAs and 292 were lncRNAs； These differentially expressed genes were significantly enriched in biological processes and pathways such as axon guidance and arrhythmogenic right ventricular cardiomyopathy； Three candidate lncRNAs （MSTRG.13015， MSTRG.7609， MSTRG.18703） might play important regulatory functions during the differentiation of porcine skeletal muscle satellite cells. MSTRG.13015 had a relatively high expression level at 60 hours of porcine skeletal muscle satellite cell differentiation. MSTRG.7609 had relatively higher expression levels at 0 hours and 48 hours of differentiation compared with that at 60 hours of differentiation. The expression level of MSTRG.18703 was relatively higher than that of the previous two lncRNAs and continued to increase with the increase of differentiation time.