Discovery and Identification of Autotoxic Substances and Transformed Strain of <i>Rehmannia Glutinosa</i> Continuous Cropping Obstacle

WANG Mingdao; CHU Mengxiao; CAO Hongli; HAO Shanghua; QIU Shuang; ZHANG Yuhang

doi:10.3969/j.issn.1003-0972.2021.02.007

WANG Mingdao, CHU Mengxiao, CAO Hongli, HAO Shanghua, QIU Shuang, ZHANG Yuhang. Discovery and Identification of Autotoxic Substances and Transformed Strain of Rehmannia Glutinosa Continuous Cropping Obstacle[J]. Journal of Xinyang Normal University (Natural Science Edition), 2021, 34(2): 208-215. DOI: 10.3969/j.issn.1003-0972.2021.02.007

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Discovery and Identification of Autotoxic Substances and Transformed Strain of Rehmannia Glutinosa Continuous Cropping Obstacle

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The blank soil and the first stubble soil were systematically analyzed and positioned by thin layer chromatography (TLC), and prepared by the microbial to transformation of Rehmannia glutinosa. It was identified as the autotoxic substance of Rehmannia glutinosa. The results showed that the substance is present in extracted by dichloromethane of the first stubble soil was ergosterol-4, 6, 8 (14), 22-tetraene- 3-one, the transformed strain was Aspergillus puniceus, named Aspergillus puniceus FF5YH.

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Discovery and Identification of Autotoxic Substances and Transformed Strain of Rehmannia Glutinosa Continuous Cropping Obstacle

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