Cloning and Bioinformatics Analysis of OsMDH Gene in Rice

OsAAP6 gene of rice (Oryza sativa L.) is a major quantitative trait locus (QTL) gene controlling rice seed protein content, which have an important impact on rice quality traits. OsMDH protein can interact with OsAAP6 gene. The physical and chemical properties, protein structure and function of OsMDH protein are predicted using bioinformatics strategies to explore the biological function of OsMDH gene.The results show that the coding sequence (CDS) of OsMDH gene in rice is 999 bp long, encoding 332 amino acids; The coding protein is a hydrophobic protein without signal structure, including two transmembrane structures. Through protein interaction analysis, it is found that OsMDH may interact with citrate synthase, fumarase, etc., and participate in the process of tricarboxylic acid cycle.