丽格海棠的组织培养与快速繁殖研究

Study on tissue culture and rapid propagation of Begonia elalior

摘要: 以丽格海棠的茎尖、叶柄、叶片为外植体进行组织培养 .最佳培养基 :(1)诱导培养基 MS+6 -BA4.0 mg/ L (单位下同 ) +IBA 1.0 ;(2 )增殖培养基 MS+6 - BA 0 .5 +IAA0 .2 ;(3)生根培养基 MS+NAA0 .2 .生根壮苗培养 5 0 d左右 ,经移栽 ,成活率达 92 %左右 .

Abstract: Tap buds,leaves and leaf stalks of Begonia elalior,after sterilized,were used as tissue culture explants.The most appropriate culture medium in culture stages were(1)MS+6 BA4.0 mg/L+IBA1.0 mg/L for germination;(2)MS+6 BA 0.5 mg/L+IAA 0.2 mg/L for rapid propagation;(3)MS+NAA 0.2 mg/L for rooting.Plantlets were transplanted after rooting culture about 50 days.The survival rate of transplanted was to 92%.