Abstract: The chemical constituents of roots from the Echinops integrifolius Kar.et Kir.were isolated by silica gel column chromatography,Sephadex LH-20,recrystallization and HPLC.The chemical structures were identified according to the physicochemical properties and spectral data.The antioxidant activity was determined by DPPH free radical scavenging assay.Fifteen compounds were isolated and nine compounds identified as daucosterol (1),protocatechuic acid (2),protocatechualdehyde (3),caffeic acid (4),scopolin (5),breynioside A (6),gentisic acid (7),5,7,4'-trihydroxy-3,6-methoxyflavone (8),centaureidin (9).The results of the DPPH free radical scavenging assay on some of the compounds showed compounds 2,3,4 and 7 had better antioxidant activity than positive control Vc.The IC₅₀ of compounds 2,3,4 and 7 were 2.177、0.844、0.800、1.434 mg/L repectivtly,positive control Vc was 3.629 mg/L.Compounds 3~7 were isolated from the genus Echinops integrifolius for the first time.Compounds 2,3,4 and 7 had good antioxidant activities.