小茴香中挥发性成分的超声雾化-固相萃取研究

Extraction of the Volatile Compounds in Foeniculum Vulgare Mill. Using Ultrasonic Nebulization Extraction Coupled with Solid-phase Extraction

摘要: 利用超声雾化- 固相萃取法萃取，气相色谱法和气相色谱- 质谱法测定了小茴香中的挥发油．采用正十四烷作为洗脱溶剂，体积为 50 μL、吹扫气流速为200mL/min，吹扫时间为 5 min．利用优化后的方法测定了小茴香中挥发性成分的种类和含量，与水蒸馏提取法所得结果相似．测定样品溶液中挥发性成分的回收率为65.8% ～102.0%，相对标准偏差小于 8.0%．

Abstract: The ultrasonic nebulization extraction coupled with solid-phase extraction (UNE-SPE) followed by gas chromatography and gas chromatography-mass spectrometry was developed and applied to extract and determine the volatile compounds from foeniculum vulgare mill. Experimental parameters, including the kind of extraction solvent (n-tetradecane), solvent volume (50 μL), flow rate of purging gas (200 mL/min) and purging time (5 min), were examined and optimized.The contents of the constituents from foeniculum vulgare mill obtained by the proposed method were closed to those obtained by hydro-distillation (HD). The recoveries for determining the four constituents are between 65.8%~102.0% and RSDs are less than 8.0%