Abstract: Acute toxicity and joint toxicity of copper, zinc on Sinocalanus dorriiare studied by single factor static acute toxicity test method and equal toxic solution method. Acute toxicity test results showed that the median lethal concentrations (LC50) of copper ions, zinc ions for 24 h to Sinocalanus dorrii were 0.481, 3.221 mg/L; LC50 for 48 h were 0.383, 1.573 mg/L; LC50for 72h were 0.315, 0.471 mg/L. The joint toxicity presented characteristics shows that highstrength of copper ions performs synergistic effect and high-strength of zinc ions performs antagonistic effect to Sinocalanus dorrii.