李丹彤, 王红, 郝杰, 曹善茂, 宋坚, 何旭颖. 三种海藻凝集素对刺参组织免疫酶SOD、PO活性的影响[J]. 信阳师范学院学报(自然科学版), 2014, 27(1): 34-37. DOI: 10.3969/j.issn.1003-0972.2014.01.010
引用本文: 李丹彤, 王红, 郝杰, 曹善茂, 宋坚, 何旭颖. 三种海藻凝集素对刺参组织免疫酶SOD、PO活性的影响[J]. 信阳师范学院学报(自然科学版), 2014, 27(1): 34-37. DOI: 10.3969/j.issn.1003-0972.2014.01.010
Li Dantong , Wang Hong, Hao Jie, Cao Shanmao, Song Jian, He Xuying. Effects of Three Marine Alga Lectins on the Activities of Major Immunoenzymes SOD and PO in Apostich[J]. Journal of Xinyang Normal University (Natural Science Edition), 2014, 27(1): 34-37. DOI: 10.3969/j.issn.1003-0972.2014.01.010
Citation: Li Dantong , Wang Hong, Hao Jie, Cao Shanmao, Song Jian, He Xuying. Effects of Three Marine Alga Lectins on the Activities of Major Immunoenzymes SOD and PO in Apostich[J]. Journal of Xinyang Normal University (Natural Science Edition), 2014, 27(1): 34-37. DOI: 10.3969/j.issn.1003-0972.2014.01.010

三种海藻凝集素对刺参组织免疫酶SOD、PO活性的影响

Effects of Three Marine Alga Lectins on the Activities of Major Immunoenzymes SOD and PO in Apostich

  • 摘要: 在基础饲料中分别添加质量分数为0.90%、1.80%、3.60%的裙带菜凝集素,1.36%、2.73%、5.45%的孔石莼凝集素,0.47%、0.94%、1.88%的萱藻凝集素,以基础饲料组为对照组,于投喂后第5、10、15、20天检测超氧化物歧化酶(SOD)、酚氧化酶(PO)活性.结果显示,各实验组的SOD、PO活性与对照组比较均有提高,且与对照组有显著性差异(P0.05)和极显著差异(P0.01).投喂1.80%质量浓度裙带菜凝集素组、1.36%质量浓度孔石莼凝集素组、1.88%质量浓度萱藻凝集素组效果最佳.海藻凝集素可作为刺参免疫增强剂使用,建议其适宜添加量为:1.80%裙带菜凝集素、1.36%孔石莼凝集素和1.88%萱藻凝集素

     

    Abstract: The effects of three lectins fromUndaria pinnatifida lectin,Ulva pertusa  lectin andScytosiphon lomentarius lectin on the activities of Superoxide dismutase (SOD), Polyphenol oxidase (PO) ofApostichopus japonicus were studied by supplying various lectin . The experimental dietary levels ofUndaria pinnatifida lectin were 0.90%(m/m), 1.80% and 3.60%, andUlvapertusa lectin were 1.36%、2.73%、5.45%, andScytosiphon lomentarius lectin were 0.47%、0.94%、1.88% respectively, Group fed with basic feed was set as control. On the 5th, 10th, 15th and 20th day after feeding, the activities of Superoxide dismutase (SOD) and Polyphenol oxidase (PO) inApostichopus japonicus were determined.The results indicated that the activities of SOD and PO of each group increased,respectively, has a significant difference(P <0.05) and a highly significant difference (P <0.01), with the control group. Feeding 1.80% concentration group ofUndaria pinnatifida lectin;1.36% concentration group ofUlva pertusa lectin;1.88% concentration group ofScytosiphon lomentarius lectin are best. The results show that marine algae lectin can be used as theApostichopus japonicus immune enhancer, it is recommended that the appropriate dosage: 1.80%Undaria pinnatifida lectin, 1.36%Ulva pertusa lectin and 1.88%Scytosiphon lomentarius lectin

     

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