Abstract: A simple spectrometric method was developed for the determination of hemoglobin based on it s catalytic activity on oxidation of alizarin red in presence of hydrogen peroxide.The optimum conditions and effects of foreign ions were investigated.The results indicated that hemoglobin concentration demonstrated good linear correlation in the range of 1.5×10-9～5.0×10-8 mol/L,and the apparent molar absorptivity ε532 is 3.4×106 L·mol-1·cm-1 with a detection limit of 6.0×10-10mol/L.This method has been applied to t...