白花泡桐叶柄愈伤组织再生植株的诱导与培养

Plant Regeneration from Petiole Calli of Paulownia fortunei

摘要: 为提高白花泡桐繁殖效率,防止丛枝病的传播,保持优良种性,利用愈伤组织再生技术对其增殖.研究表明:叶柄产生愈伤组织最适培养基为MS+2.0 mg/L BA+0.2 mg/L NAA+0.05 mg/L2,4-D;诱导芽分化最适培养基为1/2MS+2 000 mg/L脯氨酸,可使诱导分化率达27%;增殖继代最适培养基为B5+1.0 mg/L BA+0.1 mg/L NAA,增殖系数达6.79;生根最适培养基为1/2MS+0.1 mg/L IBA+0.1 mg/L NAA,生根率达100%.

Abstract: For developing propagation,preventing the spreading of Paulownia Witches'-broom(PaWB) and holding excellent variety,the technique of plant regeneration from petiole calli of Paulownia fortunei and rapid propagation was studied.The result showed that MS+BA2.0 mg/L+NAA0.2 mg/L+2.4-D0.05 mg/L was the best culture medium of callus induction.The suitable medium for regeneration was 1/2MS+2 000 mg/L proline.The regeneration frequency can be 27%.The best culture medium of propagation was B_5+BA1.0 mg/L+NAA0.1 mg/L...