Abstract: The research was made on in vitro culture of young panicles of O.rufipogon.With the development of young panicles,the induction frequencies of calli from young panicles of O.rufipogon appeared first from low to high,and then returned to low.The highest induction frequency reached 61.1%.It was necessary to add 2,4-D to mediums for inducing calli of young panicles,whereas KT and NAA were not essential.Regeneration frequencies of the second pathway were higher than those of the first pathway.