Abstract: The axillary buds' culm segments of the Yabukita were used for explants to obtain plants regeneration and erect fast asexual generation system.MS was used as minimal medium,appending phytohormone of different kinds of pH indicators.The experimental results show that the optimal inducement culture medium is MS+6BA2.0 mg/L;the sprout enrichment culture medium is MS+6BA1.0 mg/L+IBA0.2 mg/L;the optimal rootage culture medium is 1/2MS+BA0.5 mg/L+IBA0.1 mg/L+NAA0.2 mg/L+Al3+0.01mg/L.