光度法测定常见蔬菜的抗氧化活性

Spectrophotometry determination of antioxidant activity of vegetables

  • 摘要: 在稀硫酸介质中,羟自由基迅速氧化丁基罗丹明B,使其褪色.在558 nm处测定吸光度的变化,可间接测定羟自由基的生成量;蔬菜提取物可以清除溶液中的羟自由基,从而使溶液的吸光度下降程度减弱.由此建立了一种测定蔬菜对羟自由基的清除率的新方法.测试了菠菜、油菜、香菜等10种蔬菜的抗氧化活性,其中菠菜、油菜、香菜的抗氧化活性较强.

     

    Abstract: Butyl Rhodamine B was oxidized by hydroxyl radical rapidly and decolorized in dilute sulfuric acid medium.The change in absorbance , corresponding to color intensity change of Butyl Rhodamine B can be determined at 558 nm, so the amount of ·OH produced can be determined indirectly. Extractives of vegetable might eliminate hydroxyl radical in solution and further dereases the decline of absorbarce of solution.Based on above-mentioned principle, a new method was developed to determine the eliminating ratio of...

     

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