大岩桐的组培快繁和温室栽培

The rapid propagation and culture under-glassof Sinningia hybrida Hort.

摘要: 以大岩桐 (Sinningia hybrida Hort.)叶柄为外植体 ,通过 2次灭菌后进行组织培养 .适宜条件为 (1 )诱导培养基为 MS+BA2 .0 mg/L;(2 )继代和增殖培养基为 MS+BA1 .0 mg/L+NAA0 .5 mg/L;(3 )生根培养基为 MS+NAA 0 .2 mg/L或 MS+IBA 0 .5 mg/L,大岩桐在适合的栽培条件下生长良好 ,具有很强的观赏性 .

Abstract: Leaf stalks of Sinningia hybrida Hort.was sterilzed twice and used as tissue culture explants. The most appropriate media in culture stages are as follows:(1)MS+BA 2.0 mg/L for germination, (2)MS+BA 1.0 mg/L+ NAA 0.5 mg/L for rapid propagation, (3)MS+NAA 0.2 mg/L or MS +IBA 0.5 mg/L for rooting. Sinningia hybrida Hort. grew well in the good cultivation condition.