摘要:
以铺道蚁(Tetramorium caespitum)为实验材料,采用正交试验法,对蚂蚁蛋白质的提取条件进行了研究.实验分别采用碱提蛋白法、盐提蛋白法、Tris-HCl缓冲液提蛋白法等3种提取方法.对其主要条件:不同浓度、浸提温度、浸提时间、固液比以及Tris-HCl液的pH值等进行了研究.结果表明,碱提蛋白法的最佳条件为:碱液浓度为1%,固液比为1∶15,浸提时间为60min,浸提温度为80℃;盐提蛋白法的最佳条件为:盐液浓度为1.5%,固液比为1∶15,浸提时间为60min,浸提温度为50℃;Tris-HCl缓冲液提蛋白法的最佳条件为:pH值为8.3,固液比为1∶15,浸提时间为90min,浸提温度为20℃.在这3种方法中,碱提蛋白法的蛋白质得率最高,其次为盐提蛋白法,而Tris-HCl缓冲液提蛋白法最低.
关键词:
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正交法
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蛋白质
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提取
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蚂蚁
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最佳条件
Abstract:
The extract method of protein from Tetramorium caespitum was investigated by the orthogonal test. Three extract methods,alkali treatment,salt treatment,and Tris-HCl buffer treatment were used in this study,respectively,and the factors for concentration,temperature,time,the ratio of solid to liquid and the pH for Tris-HCl buffer were analyzed. The results showed:the optimized condition for alkali treatment was a solid/liquid ratio of 1∶15 in 1% NaOH solution for 60 min at 80 ℃;the optimized condition for sal...
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