Abstract: The extract method of protein from Tetramorium caespitum was investigated by the orthogonal test. Three extract methods,alkali treatment,salt treatment,and Tris-HCl buffer treatment were used in this study,respectively,and the factors for concentration,temperature,time,the ratio of solid to liquid and the pH for Tris-HCl buffer were analyzed. The results showed:the optimized condition for alkali treatment was a solid/liquid ratio of 1∶15 in 1% NaOH solution for 60 min at 80 ℃;the optimized condition for sal...