嘌呤嘧啶类化合物的反相离子对色谱分离及饮料中咖啡因的测定

Separation of Pruine and Pyrimidine Compounds by Reversed Phase Lon-Pair Chromtography and the Deter

摘要: 本文报道了以含７．５％甲醇（Ｖ／Ｖ），１０ｍｍｏｌ／Ｌ（Ｃ４Ｈ９）４ＮＢｒ，１０ｍｍｏｌ／ＬＫＨ２ＰＯ４～Ｎａ２ＨＰＯ４缓冲溶液（ｐＨ５．０）作流动相，在ＹＷＧＣ１８柱上，反相离子对色谱法同时分离胞嘧啶、黄嘌呤、腺嘌呤、可可碱、尿嘧啶丙酸、茶碱、咖啡因７种碱基及其衍生物的方法，并用于软性饮料中咖啡因的测定，标准加入回收率在９５．３％～１０４．５％之间。

Abstract: This paper reports a separation method of seven base and their derivatives including cytosine, xanthine ,adenine,cacaine,1-propionyloxyuracil,theophylline and caffeine on a YWG C18 column by reversed phase ion-pair chromatography with 7.5% CH 3OH(V/V),10mmol/L (C 4H 9) 4NBr,10mmol/L KH 2PO 4 -Na 2HPO 4(pH5.0) buffer solution as a mobile phase.This method was used for the determination of caffeine in drink,95.3%-104.5% recovery was obtained.