Abstract: An efficient solid-phase extraction column was fabricated using graphene as adsorbent.Some factors affecting the extraction efficiency were studied.Fluorescent production was produced after 8-(3’,4’-diaminophenyl)-difluoroboradiaza-s-indacene as fluorescent probe reacted with nitrite in aqueous medium at 30 ℃ for 15 min.A new spectrofluorimetry method was developed for the sensitive determination of nitrite after extracting nitrite derivative.The response was a linear function of concentration i...