Abstract: Tenebrio molitor antifreeze protein gene TmAFP84 was obtained through RT-PCR method.Then we constructed cloning recombinant plasmid pUCm-T-TmAFP84,which was identified by double restriction enzymes digestion analysis,PCR and sequencing.The sequence of TmAFP84 was compared with relative nucleotides reported in GenBank.Display: TmAFP84 shared high homology with other AFP gene of Tenebrio molitor,the value is up to 98.81%.We also constructed expressing recombinant vector pMAL-p2X-TmAFP84 for the future protein...