Abstract: Callus were induced from explants of bulbils of Pinellia ternata Breit on MS medium with IAA 3.0 mg/L +BA0.5mg/L The milky callus induced from bulbils grown quickly. The callus could differentiate into the plantlets on solid culture and into plenty of tubers on liquid shake culture on MS mediums without plant growth hormone. The survival rate of transplanted plantlet was 92% The germination capacity of transplanted tubers was 54.3%