Abstract: For the treatment of eczema has not been scientifically confirmed， 2，4-dinitrochlorobenzene （DNCB） was used to induce eczema reaction in C57BL/6 mice， and eczema mice were treated with ethyl acetate to study the disease of eczema mice related immune factors， skin pathology slices， skin cell apoptosis indicators. The results showed that the honeysuckle cleared eczema mice serum of IgE， IL-4 and IL-17 levels， the level of CD4 + immune factors was improved， the mouse eczema caused by spine hyperplasia was reduced， the infiltration of inflammatory cells and tissue edema was reduced， also the apoptosis of keratinocytes was promoted， the symptoms of eczema mice had been certain improvement. The experimental results also showed that the ethyl acetate site of honeysuckle treated eczema by inhibiting the production of inflammatory cells and regulating immune factors.