Abstract: To understand the biological role of Salvia miltiorrhiza LBD26 transcription factor in the accumulation of medicinal ingredients, the SmLBD26 gene was cloned; the sequence characteristics, homologous evolution and promoter element analysis were investigated using bioinformatic methods; The expression specificity of the tissues and the expression level under hormonal and stress treatments were tested by quantitative real time polymerase chain reaction(qRT-PCR). Sequence characterization results showed that the total length of SmLBD26 CDS was 702 bp, encoding 233 amino acids and the SmLBD26 protein was an unstable, hydrophilic nucleoprotein. The secondary structure of the SmLBD26 protein was dominated by random coil, followed by α-helix, extended strand and β-turn. The Blastp and phylogenetic analysis found that it contained a LOB domain, belonged to the Class Ⅱ LBD subfamily and it had high homology and a close evolutionary relationship with LBD proteins from other species. Expression analysis results showed that the SmLBD26 gene was expressed in roots, stems, leaves and flowers, and the expression level was high in roots. The expression of SmLBD26 was down regulated after treatment with abscisic acid, auxin, MeJA, 4 ℃ and cadmium, indicating that the SmLBD26 gene was involved in the response of Salvia miltiorrhiza to hormones and different stresses.