响应曲面优化茶树花饮料发酵工艺

Optimization of Fermentation Technology of Tea Flower Beverage by Response Surface Method

  • 摘要: 以茶树花为主要原料, 发酵制作茶树花饮料并优化其发酵工艺。以感官审评得分为评价指标, 单因素实验分析菌种、料液比、加糖量、发酵温度、发酵时间对发酵饮料的影响, 并通过响应曲面法对4个主要因素进行优化, 确定茶树花发酵饮料的最佳发酵工艺。结果表明, 料液比值1/54 g·mL-1、加糖量6.5%、发酵温度35 ℃、发酵时间41.5 h时, 得到的茶树花饮料茶香、花香、甜香最协调, 苦涩味低。主成分分析结果表明, 发酵饮料的游离氨基酸含量增加, 咖啡碱、茶多酚、儿茶素含量降低, 发酵饮料DPPH自由基清除率为85.47%。

     

    Abstract: Using tea flower as raw material, the fermented beverage was prepared and the fermentation technology was optimized. Using sensory evaluation score as evaluation index, the bacterial seed, solid-liquid ratio, sugar content, fermentation temperature and fermentation time were analyzed by single factor tests. The four factors which had great influence on the fermentation technology were optimized by response surface methodology. The results showed that the optimum fermentation parameters were solid-liquid ratio 1/54 g·mL-1, sugar content 6.5%, fermentation temperature 35 ℃, fermentation time 41.5 h. Under the above conditions, the aroma of tea, flowers and sweetness were coordinated with each other andlowest bitterness in the fermented beverage. The principal component analysis showed that the content of free amino acids was increasing, while the content of caffeine, tea polyphenols and catechins were decreasing. The DPPH scavenging rate under the optimal conditions was 85.47%.

     

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