Abstract: To enhance the production capacity of laccase in solid-state fermentation of Ganoderma resinaceum and promote the utilization of Chinese medicine residue resources, a fermentation substrate consisting mainly of Chinese medicine residue and wheat bran was employed. Laccase activity was used as the indicator for optimization through single-factor testing, Plackett-Burman (PB) test, and orthogonal experimental design. Additionally, the decolorization ability of produced laccase towards azo dyes was investigated. The results demonstrate that Ganoderma lucidum seed mass concentration of 12% into a solid fermentation medium consisting of Chinese medicinal residue (diameter 0.4 mm) and wheat bran in a ratio of 6∶4, with an initial water content of 60%, sucrose concentration of 2%, yeast powder content of 0.6%, Mg²⁺ concentration of 3 mmol/L, and initial pH value set at 5.5. After a fermentation period of 14 d ase activity reached (56.13±1.20) U/g. Furthermore, by employing media containing ABTS (2, 2'-Azinobis(3-ethylbenzothiazoline-6-sulf) Ammonium Salt), the decolorization rates for methylene blue, acid orange 7, and amino black 10B after a decolorization duration of 10 hours were found to be 46.7%, 84.6%, and 61.1%, respectively.