Abstract: To investigate the role of the CnMYB4 transcription factor in flower color and flavonoid synthesis in Camellia sinensis, the full-length sequence of CnMYB4 transcription factor as research materials was cloned. Subsequently, bioinformatics analysis was conducted. The temporal and spatial expression patterns of CnMYB4 in different tissues of Camellia sinensis were analyzed using quantitative fluorescence PCR (qRT-PCR). The results revealed that the open reading frame length of CnMYB4 was 244 amino acids. The protein contained conserved domains such as Myb_DNA binding and SANT, along with C1 (RGIDPxTHRPL/INE), C2 (PDLNLj/E LxIG/S), governmentY/F DFLGL(G), C4 (FLGLX47V/LLj/GF/YR/SX1LEMK), and conservative Motif. Phylogenetic analysis demonstrated a close relationship between the CnMYB4 protein from Camellia sinensis and CsMYB4 a protein from camellia camellia, clustering them within the same small clade. qRT-PCR analysis indicated that the expression level of CnMYB4 was relatively high in flowers but relatively low in leaves of Camellia sinensis. During opening stages, expression levels were highest during young bud stage but decreased during half-opening stage and blooming stage.