Abstract: Polyvinylidene fluoride (PVDF) composite material with electric field modulation performance was prepared by adding reduced graphene to PVDF matrix, which was further combined with silver nanowires (AgNWs) with surface plasma performance to prepare a self-powered flexible surface-enhanced Raman scattering (SERS) substrate. By the folding process, the surface of the substrate was charged, and then the Raman dye molecules with opposite charges in the solution were enriched on the surface of the substrate through electrostatic attraction. The surface plasmon resonance of the precious metal on the surface of the substrate was used to enhance the Raman signal of the dye. The morphology and structure of the self-powered flexible substrate were characterized by the scanning electron microscope (SEM), Fourier transform infrared spectrometer (FTIR), and X-ray diffractometer (XRD). The Raman enhancement properties of the substrate were characterized by the Raman spectrometer. The self-powered flexible SERS substrate integrated enrichment and enhancement, showing excellent Raman enhancement performance, and was expected to be used for highly sensitive detection of environmental pollutant molecules.