Abstract: MiR-125b belongs to the miR-125 family and is a small, highly conserved ncRNA that plays a key role in a variety of biological functions. Among them, the regulation of miR-125b on the development of animal muscle tissue (including skeletal muscle, myocardium and smooth muscle) has received extensive attention. In this review, it is summarized that the biological origin and biological function of miR-125b as well as its regulation on the development of animal muscle tissue focus on its research on the development process and disease of animal skeletal muscle, cardiac muscle and smooth muscle cells. It provides a new reference for the treatment of miR-125b in muscle diseases and the molecular improvement of meat production performance of livestock and poultry.