半夏中一株高产纤维素酶内生真菌的分离、鉴定及活性分析

Isolation, Identification and Activity Analysis of a High-yielding Cellulase Endophyte From Pinellia Ternata

  • 摘要: 以羧甲基纤维素钠为主要碳源,采用刚果红染色法从半夏中筛选出一株高产纤维素酶的内生真菌,通过形态学观察及分子生物学技术对其进行鉴定,并对该菌株发酵产物进行抑菌、抗氧化活性测定。结果表明: 高产纤维素酶的内生真菌bxg1被鉴定为曲霉属真菌(Aspergillus chevalier)。该菌株透明圈直径D与菌落直径d比值高达2.5。菌株抑菌实验发现,该菌株对供试病原菌均有抑菌效果,对革兰氏阴性菌具有较好的抑菌活性,其中对铜绿假单胞菌的抑菌圈约为1.2 cm;抗氧化实验发现,该菌株对DPPH自由基的清除能力最强,IC50值为0.056 g/L。

     

    Abstract: Using sodium carboxymethyl cellulose as the main carbon source, an endophytic fungus with high production of cellulase is screened from Pinellia ternata by Congo red staining. It is identified by morphological observation and molecular biology techniques. The antibacterial and antioxidant activities of the fermented product of the strain are determined. The results show that the endophytic fungus bxg1 with high cellulase production is identified as Aspergillus chevalier. The ratio of transparent circle diameter to colony diameter of this strain is as high as 2.5. The bacteriostatic study of the strain finds that the strain has the bacteriostatic effect on the tested pathogens, and has the good bacteriostatic activity against Gram-negative bacteria, among which the bacteriostatic circle for Pseudomonas aeruginosa is about 1.2 cm; antioxidant studies found that, the strain has the strongest scavenging ability to DPPH free radicals, and the IC50 value is 0.056 g/L.

     

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