Abstract:
Using sodium carboxymethyl cellulose as the main carbon source, an endophytic fungus with high production of cellulase is screened from
Pinellia ternata by Congo red staining. It is identified by morphological observation and molecular biology techniques. The antibacterial and antioxidant activities of the fermented product of the strain are determined. The results show that the endophytic fungus bxg1 with high cellulase production is identified as
Aspergillus chevalier. The ratio of transparent circle diameter to colony diameter of this strain is as high as 2.5. The bacteriostatic study of the strain finds that the strain has the bacteriostatic effect on the tested pathogens, and has the good bacteriostatic activity against Gram-negative bacteria, among which the bacteriostatic circle for
Pseudomonas aeruginosa is about 1.2 cm; antioxidant studies found that, the strain has the strongest scavenging ability to DPPH free radicals, and the IC
50 value is 0.056 g/L.