水稻<i>OsMDH</i>基因的克隆及其生物信息学分析

彭波; 孙晓宇; 张庆茜; 彭娟; 娄安琪; 孙艳芳; 庞瑞华; 周伟; 汪全秀

doi:10.3969/j.issn.1003-0972.2023.02.014

水稻OsMDH基因的克隆及其生物信息学分析

Cloning and Bioinformatics Analysis of OsMDH Gene in Rice

摘要

摘要: 水稻(Oryza sativa L.) OsAAP6基因是控制水稻种子蛋白质含量的主效数量性状座位(quantitative trait locus，QTL)基因，对水稻品质性状产生重要影响，而OsMDH蛋白能够与OsAAP6基因发生相互作用。利用生物信息学策略对OsMDH蛋白的理化性质、蛋白结构及功能进行预测，探究OsMDH基因的生物学功能。结果表明：水稻OsMDH基因的编码区(coding sequence，CDS)区长999 bp，编码332个氨基酸；编码蛋白为疏水蛋白且不存在信号结构，包含两个跨膜结构。通过蛋白互作分析，发现OsMDH可能与柠檬酸合酶、延胡索酸酶等发生相互作用，参与三羧酸循环过程。

Abstract: OsAAP6 gene of rice (Oryza sativa L.) is a major quantitative trait locus (QTL) gene controlling rice seed protein content, which have an important impact on rice quality traits. OsMDH protein can interact with OsAAP6 gene. The physical and chemical properties, protein structure and function of OsMDH protein are predicted using bioinformatics strategies to explore the biological function of OsMDH gene.The results show that the coding sequence (CDS) of OsMDH gene in rice is 999 bp long, encoding 332 amino acids; The coding protein is a hydrophobic protein without signal structure, including two transmembrane structures. Through protein interaction analysis, it is found that OsMDH may interact with citrate synthase, fumarase, etc., and participate in the process of tricarboxylic acid cycle.

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