Abstract: Based on the land use data of Henan province in 2000, 2010 and 2018, the total amount and composition of ecosystem service value (ESV) in each year of Henan province are calculated and analyzed. Based on the grid scale, the spatial pattern and evolution characteristics of ESV in Henan province are explored by GIS spatial analysis tools. The results show that: (1) From 2000 to 2018, the ecosystem service value of Henan province is decreased by 3.754×10⁸ yuan, which remains stable in western and southern Henan, and changes significantly in central and eastern Henan where human activities are intensive. (2) The contribution of cultivated land ecosystem is the largest in terms of ecosystem contribution, and the value of soil conservation is the largest among the individual ecosystem service values. (3) The ecosystem service value of Henan province shows obvious scale effect. Moran index is found to be the largest at the grid scale of 5 km×5 km. Based on this scale analysis, it is found that the spatial differences of ESV in Henan province are obvious, and it is affected by different scales. The spatial pattern of decreasing from the forest areas in western and southern Henan to the cultivated areas in eastern Henan and strip-shaped distribution under the influence of water system. High-high concentration areas are concentrated on most areas of western Henan, parts of southern Henan and areas of northern Henan along provincial boundaries.