一株铁线蕨内生真菌tg-1的分离鉴定及其生物活性研究

Isolation, Identification and Biological Activity of an Endophytic Fungus Strain tg-1 from Adiantum capillus-veneris L.

摘要: 采用平板分离法对铁线蕨（Adiantum capillus-veneris L.）内生真菌分离纯化，并进行分类鉴定，然后用滤纸片法进行抑菌活性测定，分析其对DPPH、ABTS、羟基自由基的清除作用。分离得到一株内生真菌tg-1，经鉴定为曲霉属真菌（Aspergillus sp.），具有良好的抑菌效果，对大肠埃希菌（Escherichia coli）与沙门氏菌（Salmonella）的最低抑菌浓度（MIC）分别为0.781 5、0.391 1 g/L，同时具有较好的抗氧化活性，清除DPPH、ABTS、羟基自由基的IC₅₀分别为0.038、0.026、0.063 g/L。

Abstract: The endophytic fungi of Adiantum capillus-veneris L. are isolated and purified by plate separation method, and then classified and identified. The antibacterial activity is determined by filter paper method, and the scavenging effect of DPPH, ABTS and hydroxyl radicals is also analyzed.An endophytic fungus tg-1 is isolated and identified as Aspergillus sp, which has good antibacterial effect, among which the minimum inhibitory concentration (MIC) against Escherichia coli and Salmonella are 0.781 5 and 0.391 1 g/L, respectively, and have good antioxidant activity. The IC₅₀ for scavenging DPPH, ABTS, and hydroxyl radicals are 0.038, 0.026 and 0.063 g/L, respectively.