Abstract: Using 9 representative plants with whole-genome data, the evolutionary mechanism and functional differentiation of LHT gene family are analyzed in angiosperms, and the functions in soybean is also discussed through bioinformatics and molecular biology methods. The results show that a total of 114 LHTs are found in nine plant species, and 25 LHTs are found in soybean with the number of introns ranging from 1 to 9. Based on multiple sequence alignment and phylogenetic tree analysis, it is divided that the 114 LHT proteins into Ⅰ and Ⅶ groups, group Ⅱ has the most members, and group Ⅲ is specific to the monocot plants. The number of encoded the soybean amino acids ranged from 56 to 543, the relatively molecular weight ranged from 6.3×10³ to 57.1×10³, and the theoretical pI ranged from 6.03 to 9.51, all of which are transmembrane proteins.The expression pattern analyses show that the expression of GmaLHTs is induced by rhizobium infection, and some members are specifically expressed in roots and nodules after rhizobium infection.The differential expression patterns of LHT genes in different evolutionary groups in soybean suggest that it maybe undergone selection in the process of evolution. The expression level of GmaLHT17 mRNA in overexpression transgenic plants is 13.6 times higher than that of the control plants, and the number of nodules in the overexpression transgenic plants is significantly higher than that in the control plants.