Abstract: WO₃ nano-powders are prepared via the direct calcination of ammonium tungstate and used as the supports to make a series of Ru nanoparticles (NPs) with an adsorption-chemical reduction strategy. The resulting catalysts are characterized by various techniques, such as X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy, scanning electron microscopy and transmission electron microscopy. The catalytic performance of the obtained Ru NPs is assessed for the hydrolysis of ammonia borane (AB), and the corresponding reaction kinetics is explored. The results show that the optimal mass fraction of Ru loading is 2.0%. The excellent catalytic performance of Ru/WO₃ catalysts is ascribed to the porous structure, oxygen deficiency of WO₃ and high dispersion of Ru NPs. The reaction order of AB hydrolysis which catalyzed Ru/WO₃ is 0.67 and 0.87 with respect to the concentration of Ru and AB, respectively, with apparent activation energy of 34.93 kJ/mol. Upon the addition of appropriate NaOH basic solution, the turnover frequency reaches to 445.1 min^-1.