Abstract: Using the petals of Camellia japonicae as research materials, the full length sequence of LCYb is cloned by homologous cloning and RACE technology, named CnLCYb. Subsequently bioinformatics analysis of the CnLCYb is performed, and its expression pattern is analyzed at the transcriptional and protein levels. The results show that the length of open reading frame of CnLCYb is totally 1 515 bp, and encods 504 amino acids, which has many onserved domains such as PLN02463, caroteneCyCl and lycopene_CyCl. Phylogenetic analysis show that the CnLCYb protein is closely related to the LCYb protein of Camellia sinensis. The results of real-time fluorescence quantitative PCR analysis show that the expression level of CnLCYb is relatively low in young bud, early bud and chromogenic stages of flowering of Camellia nitidissima, but high in the half-opening and blooming stage, and the expression pattern of CnLCYb in the petals shows an overall upward trend.