一测多评法测定不同产地巴戟天中5种蒽醌类成分的含量
Determination of Five Anthraquinones in Morinda Officinalis from Different Producing Areas by QAMS
-
摘要: 利用高效液相色谱法, 建立同时测定巴戟天中5种蒽醌类成分含量的一测多评法。采用Venusil MP C18色谱柱(4.6 mm×250 mm, 5 μm), 流动相: 乙腈(A)-0.3%磷酸溶液(B), 梯度洗脱(0~40 min, 38% A, 40~50 min, 38% A→55% A; 50~70 min, 55% A→60% A), 流速: 0.8 mL/min, 柱温: 30℃, 进样量: 10 μL, 检测波长: 266 nm, 以2-羟基-1-甲氧基蒽醌为内参物, 建立巴戟天中其他蒽醌类成分与2-羟基-1-甲氧基蒽醌之间的相对校正因子, 并计算其含量。结果显示, 相对校正因子具有良好的耐用性, 相对标准偏差(relative standard deviation, RSD)值均小于2.00%;一测多评法与外标法所测结果无明显差异, RSD值均小于3.00%。Abstract: A method for the determination of five Anthraquinones in Morinda officinalis is established by high performance liquid chromatography (HPLC).The chromatographic separation is performed on Venusil MP C18 column (4.6 mm×250 mm, 5 μm) with the mobile phases consisting of acetonitrile (A)-0.3% phosphoric acid (B) and gradient elution (0~40 min, 38% A, 40~50 min, 38% A→55% A; 50~70 min, 55% A→60% A) at the flow rate of 0.8 mL/min.The column temperature, injection volume and detection wavelength are set at 30℃, 10 μL and 266 nm, respectively.HPLC is used to establish the relative correction factors of other components with the reference substance 1-methoxy-2-hydroxy anthraquinonoid.The results show that the relative correction factor had good durability and relative standard deviation (RSD) values are all less than 2.00%.There is no significant difference between the QAMS method and the external standard method, and the RSD values are all less than 3.00%.
下载: