雷公藤红素治疗类风湿关节炎的组学联合分析

Omics Analysis of Celastrol in the Treatment of Rheumatoid Arthritis

摘要: 用雷公藤红素处理成纤维样滑膜细胞, 通过RNA测序、定量蛋白质组学、分子对接、qPCR和Western blot检测和验证, 筛选到差异基因和蛋白质, 获得7 803个基因和3 372个蛋白质, 雷公藤红素作用机制与氧化应激和氧气水平高低相关, 也涉及细胞组分和细胞外基质蛋白的稳定和合成通路。结果显示, 雷公藤红素可通过这些机制来抑制类风湿关节炎的发病。

Abstract: Fibroblast-like synoviocytes (FLS) from RA patients were treated with celastrol, and the differential expression of genes and proteins were screened by RNA sequencing, label-free relatively quantitative proteomics, molecular docking, qPCR, and Western blot. 7 803 genes and 3 372 proteins were obtained. Celastrol was associated with significant changes in genes that respond to oxidative stress and oxygen levels, as well as genes that stabilize or synthesize components of the extracellular matrix. These results suggest that celastrol may inhibit inflammation in RA through these mechanisms.