Abstract: Based on the Hidden-Markov model searching and sequence alignment, 17 STRs (CsSTR1~17) were identified from the tea plant genome. Physicochemical analysis showed that CsSTR proteins was 147 to 552 amino acids in length, with molecular weight from 15.6 to 60.8 kD, and isoelectric point from 4.65 to 10.88. Except CsSTR3, 4, 9, 10, 11 and 13, the other CsSTR proteins were hydrophilic. Phylogenetic analysis showed that CsSTR3, 4, 10, 11, 12, 13, 14 were located in the same evolutionary branch as the STRs from Rauvolfia serpentine, Rauvolfia verticillata, Catharanthus roseus and Camptotheca acuminata, respectively. However, the number and length of CsSTR gene introns and the number and conserved CsSTR protein motifs were different among CsSTR genes with close evolutionary relationship. Expression analysis showed that CsSTR gene expression was regulated by low temperature, drought and salt stresses and methyl jasmonate, most CsSTR genes were highly expressed in apical bud, mature leaf and root. Cis-elements related to growth, development and stress response were abundant in the promoter regions of CsSTR genes. ERF, MYB, Zinc Finger, and bZIP transcription factors were protein families which had lots of binding sites in the CsSTR gene promoters.