Abstract: By using the Hidden-Markov model searching and homologous alignment methods, 108 NAC members (CsNAC) were identified in tea plant genome.For all CsNAC members, physicochemical parameters, evolutionary relationships, gene structures, and conserved motifs were investigated.Results showed that the amino acid size, molecular weight and isoelectric point of CsNAC proteins varying from 134~1950 a.a., 15.4~222 kD, and 4.60~9.91, respectively, and CsNAC3, 8, 14, 23, 32, 74, 82, 90 and 106 had transmembrane domains.The grand average of hydropathy value indicated that all CsNAC proteins were water soluble, except the CsNAC97 protein. CsNAC family was divided into 18 subclasses by evolutionary relationship analysis.ONAC022 and NAP subclasses had the most members, while AtNAC3 and OsNAC8 had the fewest members. The promoter region of CsNAC gene is rich in cis-elements in response to drought, low temperature, salt stress and pathogen infection.Transcriptomic data analysis showed that CsNAC gene family members displayed significant differential expression patterns in different organs of tea plants and under various stress treatments.Under cold and drought stresses, ABA involved in regulating the expression of key genes in the CsNAC co-expression network. KEGG analysis of genes in the co-expression network revealed that pathways as signal transduction, carbonhydrates metabolism, plant-pathogen interaction, etc, were significantly enriched.