Abstract: An ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometer (UPLC-Q-TOF/MS) method was developed for the determination of lyophilized aronia powder. The separation was performed with a gradient elution and the mass spectrometry data was collected in both ESI positive ion and ESI negative ion modes. Moreover, the acquired data was compared with the database of the characteristic ions of targeted molecules, such as the exact mass and secondary fragmentation information. The Unify 1.7 software was used for the rapid non-targeted screening of polyphenolic components without reference standards.The results showed that more than 60 polyphenols in ESI positive ion mode and more than 200 polyphenols in ESI negative ion mode were detected, respectively, and the mass deviation was less than 3 mDa.