Abstract: Taking the plateau area of Northwest Guizhou in typical karst area as the research object, based on the landscape ecology theory, and three remote sensing images of 1995, 2005 and 2015 were selected, which the landscape pattern index was used to quantitatively analyze the dynamic changes of the plateau wetland landscape pattern in the past 20 years. The results showed that: (1) Great changes had taken place in wetland landscape types in northwestern Guizhou plateau, and the total area of wetland landscape types showed a downward trend. (2) Among the wetland landscape types in the study area, the area of wetland landscape types such as rivers, reservoirs, ponds and beaches decreased seriously, while the area of wetland landscape types such as paddy fields and lakes increased due to human activities and policies. (3) Except for beach landscape types, NP and PD of other wetland landscape types increased in varying degrees. The LPI of paddy field landscape types remained basically unchanged, while that of other wetland landscape types increased in varying degrees. According to the average patch area (AREA_MN), the change of river landscape type was the most serious. (4) In the past 20 years, the heterogeneity of wetland landscape in the study area had increased, and different landscape patches tended to be discrete in space. Among them, from 1995 to 2015, the landscape spread index (CONTAG) in the study area showed a downward trend, while SHDI and SHEI showed a continuous increasing trend.