树莓多糖绿色合成纳米银颗粒的表征及其抗氧化活性研究

Characterization of Silver Nanoparticles Biosynthesized using Crude Polysaccharides of Rubus idaeus and Their Antioxidant Activities

摘要: 从树莓果实中提取的树莓多糖和硝酸银反应合成多糖纳米银颗粒（（PAgNPs），通过紫外-可见光（UV-Vis spectrum）全波段扫描、扫描电镜（SEM）以及X射线衍射（XRD）对其结构进行表征，并做抗氧化活性测试.结果表明，纳米银颗粒在435 nm处有最大吸收峰，经电镜检测多糖纳米银颗粒平均直径在20~32 nm.同时经抗氧化活性测试，对1，1-二苯基-2-三硝基苯肼（DPPH）和2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）自由基半数清除浓度（IC₅₀）分别为为64.38 mg/L和75.86 mg/L.

Abstract: Silver nanoparticles (PAgNPs) were green synthesized using crude polysaccharides from Rubus idaeus.extracts and AgNO₃ solution. The obtained PAgNPs were characterized by UV-Vis absorption spectrum,scanning electron microscopy (TEM) and X-ray diffraction(XRD). Also, the antioxidant properties of the obtained PAgNPs were assessed in vitro, using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzthiazino-6-sulphonic acid) (ABTS) radical cation decolorization test. The result indicates that the obtained PAgNPs regular spheres with an average size of 20~32 nm and a maximum absorbance at 435 nm. Moreover, PAgNPs exhibited a high radical scavenging activity for DPPH and ABTS⁺, half radical scavenging activities(IC₅₀) of 64.38mg/L and 75.86mg/L, respectively.