Abstract: A rapid and label-free fluorescent sensing method has been established for sensitive detection of tetracycline (TC) by using bovine serum albumin (BSA) templated CuNCs as fluorescence probes. The strategy permitted sensitive detection of tetracycline in a linear range from 5.0×10^-8 to 4.0×10^-5 mol/L and with a detection limit of 5.0×10^-9 mol/L. The sensor was successfully applied to determinate the tetracycline content in milk samples. It could provide a rapid and convenient fluorescent platform for tetracycline detection.