Abstract: Acetaminophen electrochemical sensor for acetaminophen was prepared by modifying the glassy carbon electrode with methyl blue and acetylene black nanomaterials. The electrochemical behavior of acetaminophen on this sensor was investigated by cyclic voltammetry. The results indicated its good electrocatalytic activity on the redox reactions of acetaminophen. Compared with the bare glassy carbon electrode, the oxidation peak current of acetaminophen on oursensorthe sensor, methyl blue and acetylene black modified glassy carbon electrode, significantly increased. Analysis was conducted by differential pulse voltammetry. The acetaminophen oxidation peak currents of acetaminophen showed a good linear relationship with and its concentrations in the range of 2.0~400 μmol/L. showed a good linear relationship with the correlation of was 0.992. The detection limit of acetaminophen was as low as 0.6 μmol/L at signal-to-noise ratio of 3. This sensor was also employed for practical detection of acetaminophen tablets with the recovery recoveries rate between 99.3% to 103%.