Abstract: WildChrysanthemum indicum, Mentha haplocalyx and Ligusticum sinense decoction was used for the preparation of compound prescription of traditional Chinese medicine in the experiment. The bacteriostasis experiment was carried out by the drug sensitive paper method, and the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were measured by two times dilution method and the nutrient agar diffusion method. Finally, the survival curve reflected by the time change was measured by the photoelectric turbidimetric counting method. The results showed that the bacteriostasis of the decoction had obvious bacteriostasis, the MIC of Escherichia coli was 250 g/L, and MBC was 500 g/L; when the concentration of the liquid was larger than 125 g/L, the decoction of traditional Chinese medicine had obvious bacteriostasis effect on Escherichia coli.