黄缘闭壳龟微卫星分子标记的开发及遗传多样性分析

Isolation and Genetic Diversity of Microsatellite Molecular Markers in the Yellow-margined Box Turtle (Cuora flavomarginata)

摘要: 采用FIASCO法构建了黄缘闭壳龟基因组微卫星富集文库，开发了18个具有多态性的微卫星分子标记.18个位点的等位基因数目为2~14个不等，每个位点平均等位基因数为6.3个；观测杂合度在0.032和0.936之间变化（平均值为0.329），期望杂合度在0.148和0.903之间变化（平均值为0.635）.黄缘闭壳龟微卫星分子标记的开发，将为其遗传学和种群结构分析研究提供有力的分子工具，并为制定新的保护策略提供理论依据.

Abstract: The 18 polymorphic microsatellite markers were developed and characterized for C. flavomarginata by using the Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO) protocol to construct an enriched library of microsatellite. These markers revealed 2 to 14 alleles, with an average of 6.3 alleles per locus. The expected and observed heterozygosities ranged from 0.032 to 0.930 (mean 0.329) and from 0.148 to 0.903 (mean 0.635), respectively. Therefore, these markers would be useful tool for future genetics and population structure studies and establish new conservation strategies of C. flavomarginata.