Abstract: The physical fitness and body compositions of 356 Han nationality women aged 50~70 years old in Nanyang area were measured using body composition analyzer and Livers were examined by type-B ultrasonic in order to explore the relationship between non-Alcohol Fatty Liver Disease (NAFLD) and the body composition changes in premenopausal and postmenopausal women. The results showed that the obesity rates of premenopausal women (PEW), 5 years after menopausal women (AM5) and 10 years after menopausal women (AM10) were 24.5%, 31.4% and 28.4%, respectively. Furthermore, the body fat, visceral fat and fat ratio of obese women were all higher than those of normal women in the same period (P<0.01).The prevalence of NAFLD of obese women in PEW, AM5, AM10 were 26.9%, 51.4% and 34.2%, respectively, which was higher than that in normal group (P<0.01). Furthermore, obesity is a risk factor for the prevalence of NAFLD in menopausal women. Compared with PEW, waist to hip ratio (WHR) of AM5 and AM10 increased, including women with NAFLD and healthy women. And the prevalence of NAFLD was correlated with the Waist circumference (WC) and WHR. Menopause women have high obesity rates, and WC and WHR can be used as reliable indicators for predicting the prevalence of NAFLD.