基于二硒化钼纳米球和杂交链式反应灵敏检测microRNA

Sensitive Detection of MicroRNA Based on MoSe₂ Nanospheres and Hybridization Chain Reaction

摘要: 利用SiO₂纳米球为模板水热法合成了二硒化钼纳米球，并以此材料为电极基底，电沉积上AuNPs后，进行交联链式反应进行信号放大，并结合过氧化物酶催化H₂O₂+HQ底液产生电化学信号高灵敏检测microRNA-21.结果表明：新方法在1.0×10^-16~1.0×10^-10 mol/L的浓度范围内呈良好的线性关系，检出限为0.16 fmol/L （R_SN=3）.该传感器被成功应用于血清样品分析.

Abstract: MoSe₂ spheres were prepared by a simple hydrothermal method using SiO₂ as template and as electrodes material, and applied as a sensing platform after electrodeposition of AuNPs on it. Hybridization chain reaction was used to amplify signal and HRP catalyzing H₂O₂+HQ system was used to produce electrochemical response for detection of microRNA-21. New method showed a good linear relationship ranging from 1.0×10^-16 to 1.0×10^-10 mol/L with a detection limit of 0.16 fmol/L (R_SN=3). The proposed biosensor was successfully applied for serum analysis.