Abstract: Wnt/β-catenin signaling pathway has important functions in regulating gene expression and DNA damage response(DDR). Oxidative stress, which is responsible for majority of DNA lesions, affects Wnt signaling. Firstly,the Wnt/β-catenin signaling pathway and DDR were summarized, then the role and mechanism of Wnt/β-catenin signaling in DDR and oxidative stress were reviewed, in the hope of providing more effective strategies for treatment of cancer cells with deficient DNA repair capacity.