Abstract: A series of PPG-DMPA-IPDI waterborne polyurethane (WPU) were synthesized by using polypropylene glycol (PPG-2000), 2, 2-dimethylolpropionic acid(DMPA)and isophorone diisocyanate (IPDI) via an one-pot reaction and a high shear emulsification method. The effects of the DMPA content on the Zeta potential and the particle size distribution of the waterborne polyurethane were studied, and the WPU were characterized by the Fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), thermal analysis (TGA-DTA). The results showed that the WPU exhibites optimal dispersion stability at the neutralization degree of 100%, NCO/OH molar ratio (R) of 3.7, and the DMPA amount of 5%~8%. With the decreasing of DMPA amount, the particle size of WPU increases whereas their thermal stability and degree of microphase separation decreases. In addition, by using the one-pot reaction and the high shear emulsification method, the reaction time is shorter than that using the step-wise reaction and the high speed stirring emulsification method.